Recovery of isolated pancreatic isletsderived from gut leak-induced diabetes type ii mouse model

Abstract

Gut leak in the obese patient was known to be one of the predisposing factors causing diabetes type II. Indeed, the interleukin-10 (IL-10) plays a key role in gut mucosa homeostasis preventing the gut leak. Nevertheless, the linkage of diabetes type 2 caused by gut leak with IL-10 deficiency in the non-diabetes patient has not been investigated yet. Islets transplantation is known as the best alternative treatment for diabetes. The culture of isolated islets is still facing difficulties to preserve the morphology, viability, and functionality. Therefore, the present study was aiming to prove the concept of gut-leak on IL-10 deficient mice inducing diabetes type II and aiming to validate the way to preserve and recover the diabetes type II isolated islets using the newly established conditioned media. The 13-week-old age mice were separated into three groups (n=6/group) including background wildtype control (WT), transgenic control (IL-10KO), and gut leak-induced diabetes type II (GL-IL-10KO) which were induced by gavaging the dextran sulfate sodium (DSS). Then, mice were sacrificed, and the islets were isolated after 12 weeks nurtured and checked for morphology, viability, and functionality. The WT islets were used for validating two newly established conditioned media, VSCBIC-1 and VSCBIC-2, by using RPMI1640 as the basal and control medium. The GL-IL-10KO showed a defect in the viability and functionality of the isolated islets after isolation. Additionally, VSCBIC-1 was chosen as the best newly established conditioned media for preserving the WT isolated islets at least for 21 days. Then, VSCBIC-1 was used for culturing the GL-IL-10KO islets, and the results showed that the GL-IL-10KO could catch up the IL-10-KO and WT in term of viability. The functionality among GL-IL-10KO, IL-10-KO, and WT islets was upsurged gradually from day 0 to day 21. In brief, the GL-IL-10KO shows the defect on islets efficacy. Moreover, the newly established conditioned media VSCBIC-1 was able to recover the damaged islets in vitro.